I wanted to separate alkaline protein from the whole blood. I am in a confusion that heparin or EDTA vacutainer to use. So please give me a solution with an explanation to your answer.
I have placed IMU MPU2950 on an AMR (Autonomous Mobile Robot) with a metallic body. Even though I am using Madgewick Filter to smooth out the data, it is not enough and confuses the localization...
13 May 2022 1,228 3 View
I know I should be using the concept of Dead Reckoning. But how do I use prediction algorithms to determine/estimate the 2D coordinates and yaw angle, if I can get the gyroscope data, wheel...
06 May 2022 2,968 3 View
title - did covid-19 effect in import, export and GDP data - time series country - only one country dependent variable - GDP independent variable - import, export dummy - pandemic crisis Then I...
08 October 2021 4,153 4 View
Hi I need to model a Polycarbonate base adherend and on top of it we Injection mould a Polyamide material(Which acts as adhesive) and sticks very well to the polycarbonate(refer attached image)....
18 July 2021 5,518 1 View
Distance learning is a learning method in which students are not required to be physically present in a classroom setting or school.
25 December 2020 8,704 10 View
Some of the problems faced by farmers..... 1. Poor marketing channel 2. Farmers not getting remunerate price for the own produce 3. Lack of bargaining power (someone fix the price) 4. Monsoon...
30 November 2020 3,819 4 View
Based on my perception, organic farming we can use organic based fertilizers like biofertilizers, Azolla, Panchakavya, Neem oil extract etc. Whereas, in natural farming no application of any kind...
23 November 2020 7,080 22 View
In India, According to the latest National Crime Records Bureau (NCRB) data on suicides, 10,281 farmers committed suicide in 2019, down from 10,357 in 2018, whereas the figure for daily wagers...
19 November 2020 964 9 View
Hi, Is it necessary to Qualify the operator who is going to sample after cleaning validation for checking the equipment "visually clean"? If, have to qualify the vendor what are the...
17 May 2020 7,409 0 View
Can anyone help me to differentiate between OEL and PDE? Is it possible to calculate PDE value with OEL?
21 April 2020 9,433 8 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View