Hey Sashi

Look these links

Structural and functional characterisation of cardiac fibroblasts ...

cardiovascres.oxfordjournals.org/content/65/1/40

by P Camelliti - ‎2005 - ‎Cited by 556 - ‎Related articles

Jan 1, 2005 - Impact factor 5.465 ... Cardiac fibroblasts form one of the largest cell populations, in terms of cell numbers, in ... This is observed in ischemic and rheumatic heart disease, ... A more specific marker for cardiac fibroblasts is the collagen ... on cardiomyocytes or cardiac endothelial and smooth muscle cells [9].

Three-Dimensional Paper-Based Model for Cardiac Ischemia The ...

https://dash.harvard.edu/bitstream/handle/1/.../Mosadegh_ThreeDimensional.pdf?...1

by B Mosadegh - ‎2014 - ‎Cited by 28 - ‎Related articles

May 21, 2016 - Keywords: Cardiac ischemia, 3D cell culture, Cardiomyocytes, Co-culture, ... chemokines that subsequently trigger fibroblasts residing in .... cell, or co-cultures of more than one type of cell). iv) Wax-printed .... ~30% between day 2 and day 7, and suggests ischemia-induced injury is gradual under these.

good luck

Thank you Awatif,

I actually read the first article before, that is what confused me more. Do you have any personal experience with these cells?

I always that, Cardiomyocytes are the primary targets of the ischemia-reperfusion, and they are more vulnerable to oxidative distress, once they start dying and cause inflammation, then the infiltrating leukocytes/lymphocytes activate fibroblasts to cause cardiac fibrosis.

Any thoughts on this?

Thanks for the second paper, it is very interesting.

Sashi

Keep us informed if you compare the response in vitro cardiomyocyte Versus cardiac fibroblasts, to hypoxia / reoxygenation. It would be interesting to compare both cell type for cell lysis, apoptosis, mitochondrial activity. Maybe it has already been done, I do not know if this methodology would be correct. In addition, it remains of cell cultures, the phenomena can be quite different from an in vivo organ.

I would like to test that but I have not developed cultured cardiac fibroblasts... sorry ;-)

Damage of what cell type impacts cardiovascular dysfunction to a greater intensity?

I think we must consider the long-term effects (other than the direct short-term effects of ischemia-reperfusion.) :

- cardiac fibroblasts play an essential role in the fibrosis and remodeling by increased proliferation and elevated collagen production. Cardiac fibroblasts account for 60 - 70% of the cells in human heart !

- cardiomyocyte hypertrophy... after neighboring cardiomyocyte death.

The two phenomena are closely linked!

Not easy to quantify the proportion of cardiomyocytes vs. fibroblasts affected in this context, in short-term or long-term observation...