How to plot data in 10X10 matrix in which all the diagonal elements are 0 and the matrix is symmetric about the diagonal?

I have a data of comparison of 10 proteins with each other under two parameters i.e 100 entries in the shells of two 10X10 matrix in excell sheet. I want to see whether both of these data sets...

02 March 2020 1,593 5 View

What does it mean by energy band convergence in semiconductor by doping?

If band structure is anisotropic in k-space, then how will the convergence of two bands normally appearing at two different symmetric point in the Brillouin zone will enhance See-back Coefficient?

04 February 2020 1,916 3 View

Does it require testing each topical formulation of our drug in laboratory animals, if drug has showed potential at some concentrations?

We have evaluated the wound healing potentials of a compound at a particular concentration in ointment form in laboratory animals. Now, we are preparing different types of topical formulations...

14 January 2020 2,893 6 View

Problem in downloading particular ZINC database file, suggestions?

Can anybody provide me with underneath Zinc database (.sdf format) https://zinc.docking.org/substances/subsets/biogenic/ or suggest me how to download this database. Although, I have tried many...

27 November 2019 7,112 8 View

What are the different Nonclinical Pharmacological/Toxicological studies required for the development of Topical Drugs for wound healing?

I am testing some compounds for wound healing in rats (rodent) and want to further extend the research for the drug development for wound healing in animals/human. So, please tell me the different...

30 March 2019 8,252 3 View

How to calculate average contact pressure of actual contact area in COMSOL?

Hello, Im simulating currently contact pressure in COMSOL 5.3a. For that i want to know the average contact pressure for every time step. I know that you can calculate the surface average, but my...

13 February 2019 3,249 1 View

I am evaluating Graft V Leukemia in chimeric mice - how long should I wait to observe significant rejection of leukemia in the mice?

Hello everyone, I am evaluating the graft vs leukemia effect in chimeric mice. The chimeric mice were created by transplantation of hematopoeitic stem cells (H2kb) of wild type mice into BALB/c...

09 February 2016 9,026 4 View

Can we blot the membrane for two different proteins by using specific monoclonal antibodies from the same origin/species after mixing both together?

Blotting the same membrane for two different protein saves the time and cost. There are many ways of doing this either with stripping or without stripping. Which is the better approach and can we...

12 January 2015 302 2 View

Which company/brand provides good quality Trizol or Ribozol for RNA isolation from skin tissues?

21 August 2014 2,107 5 View

No title

19 August 2014 7,192 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Suggest me some trending topics for phd research in text mining/AI/machine learning?

My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.

24 February 2021 393 14 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View