I have trouble finding in vitro data on the efficiency of cosmetic treatments. Do they rather use cell lines or primary cells ? Such as adipocytes or hepatocytes cultures ?
normally keratinocytes and fibroblasts.
of late SVF derived stromal cells or ADSC are used in combination with PRP.
Also Melanocyte culture to treat Vitiligo
Fibroblast (e.g. ATCC® CRL-2703™), Keratynocites (e.g. ATCC® PCS-200-010™ or ATCC® PCS-200-011™), Melanocytes (e.g. ATCC® PCS-200-013™), BALB/3T3 clone A31 (e.g. ATCC® CCL-163™)
I am looking for information about the typical error obtained when you count corneal endothelial cells using specular microscopy (in number of cells/mm²). Can you help me ? I found around 5% of...
06 July 2016 2,805 2 View
Hi ! I wanted to do flow cytometry on primary adipocytes and hepatocytes but I cannot find any publication with protocols. Is it possible to do on primary cells ?
09 October 2015 6,214 4 View
Hi ! I am working on 3D culture of cancer cells forming spheroids in hydrogels for drug discovery and screening. Do you think that these small tumors are the best model for testing anti-cancer drugs?
09 October 2015 7,879 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View