In our work, the pus in the container was aspirated from renal pelvis by a surgeon in a patient from our research. The research which was done by a team work with me aimed to detect formation of microbial biofilm on double J stent catheter in ureter. During surgery, the surgeon was aspirated this huge amount of pus. i would like to show your suggestion about the type of infection either single or mixed bacterial infection and tpoe of bacteria.