Currently we are using fruits and vegetables waste material for the production of fungal cellulase production, particularly banana peels. However, now we are selecting substrates for yeast fermentation. Any suggestions?
You may combine any pretreated straw with wheat bran. if you use some fruit peels it is possible that the fungus may divert energy to pectinases production decreasing cellulase levels.
You may used carboxymethylcellulose (CMC) as a substrate because most of the reports showed it induced the production of cellulases by activating cellulose regulatory protein called the cellulase activator molecule (CAM)
Well, in our hands CMC is a really poor source for cellulase production; I would not recommended it. Pretreated lignocellulose residues alone or in combination with wheat bran are good choices. You may also add Twee 80 for better results.
Thanks everyone for your insights. We have tested apple peels, banana peels, corn cob, sugarcane-bagasse, grass for fungal fermentations. Moving towards yeast fermentation, particularly under SSF seems a bit tricky. But I got a number of useful suggestions through this post. In our case, CMC proved to be an inducer for endoglucanase along with co-induction of xylanase in few species. Instead of using CMC as a sole substrate we supplement the crude substrate with it to study the induction if there is any,
Lignocellulosic material in most fruit peels is commonly less than other type and part of plant eg. rice straw, bagasse etc. CMC is the best choice, but you also can try commercial cellulose. Addition of surfactant like Tween-80/20 might be useful in promoting the release of cellulase.
Banana peel is a good cellulase inducing substrate as I also worked on that but in case of bacterial cellulase. For yeast you can choose 'sugarcane bagasse' as it is a good inducer of cellulase and definitely you will get the most. All d best.