Simpler substrates like carboxymethyl cellulose(CMC), avicel or substrates like rice straw, sugarcane bagasse can give cellulase with better activity. Rice straw and similar substrates have lignin as a barrier, so does it have any effect for its activity?
in order to increase the final yield of your enzymatic reaction, the enzyme should has direct asses. In this case its important to pre-treat your substrate......
I agree with James. Note that CMC is a very good substrate for endoglucanases, but that it is not attacked by cellobiohydrolases. Many cellobiohydrolases will digest chromo- or fluorogenic cellobiosides, like p-nitrophenyl-beta cellobioside or methylumbelliferyl-beta-cellobioside, which make for easy assays. In addition, cellulase sytems need a means to remove cellobiose, which is an inhibitor of cellobiohydrolases. This can be achieved either by uptake systems, which internalize cellobiose and cellodextrins, or by betaglucosidases, which hydrolyze cellobiose ito glucose
Cellulase is a complex of enzymes, which work togather. Several different kinds of cellulases are known, which differ structurally and mechanistically. Some example are carboxymethyl cellulase (CMCase), avicelase, FPase, cellobiase and endo-1,4-beta-D-glucanase. I agree with Dr. Pierre. Besides that you could also calculate the Km and Vmax of your specific cellulase component. This will distinctively answer about the enzyme kinetics.
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