SCID mice lack T-cells. NOD mice lack B-cells. NOD/SCID lack both. NOD/SCID/Gamma (NSG) mice also lack NK cell cytotoxicity, so they are the preferred host for xenograft experiments. nude mice have more of their (mouse) immune response remaining than do the NOD/SCID or NSG mice. If you want to mimic the human immune response to your tumor and treatment agent(s), humanize the NSG mouse wth human bone marrow (thereby gaining a human immune system) and then after this has been established, inject your tumor and subsequently after it has been established (i.e., has survived the human immune response), then test your drug(s).

NSG is better choice.

NSG have been the preferred model in assessing cancer immunotherapy since these animals lack both B and T lymphocytes.130 These mice are not only transplantable with human tumors, but they also can be reconstituted with human lymphocytes to mimic immune response

This article can help you

http://www.ncbi.nlm.nih.gov/books/NBK20822/

http://bloodjournal.hematologylibrary.org/content/100/9/3175.full.pdf

thanks.

Nagarajan: Will it have any effects on IVIS?

I hope it will not have any effects on IVIS

Ic.. Cos sometimes the fur may cause a false postive

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0016700

pleas see this article

Nude mice would be your best bet. You can do an efficacy and tox study combined in a nude xenograft sudy

SCID mice (no t or b cells) are the mice of choice. Charles Mills, Ph.D.

Nudes or SCIDs are preferred mice in oncology efficacy studies. Depends on the take rate of the specific cell line. Some work best in SCIDs, some in nudes. Bothshould be able to be viewed in IVIS

SCID mice lack T-cells. NOD mice lack B-cells. NOD/SCID lack both. NOD/SCID/Gamma (NSG) mice also lack NK cell cytotoxicity, so they are the preferred host for xenograft experiments. nude mice have more of their (mouse) immune response remaining than do the NOD/SCID or NSG mice. If you want to mimic the human immune response to your tumor and treatment agent(s), humanize the NSG mouse wth human bone marrow (thereby gaining a human immune system) and then after this has been established, inject your tumor and subsequently after it has been established (i.e., has survived the human immune response), then test your drug(s).

Thanks

I´ll carry out the experiments around your tumor line development (growth, angiogenesis, metastasis,... ) by using Balb/c nude, SCID, NOD, NOD/SCID or NSG mice. The strain you finally use will depend on the immunologic status that best fits with the aim of your study. Previous comments/literature sent by other colleagues could help you a lot in this task . During these studies some toxic effect of your test compound could be detected (if any) but an aditional experiment using a immunocompetent strain (e.g. swiss, B6,) should be considered because immunodeficient strains have lymphoid tissues alterations, such partial or total depletion of thymus, spleen, lymph nodes, that could hide potential toxic effect of your drug. As you probably know, toxicity studies of chemical compounds in animals are well regulated through specific guidelines/laws (e.g. FDA, OECD,..) and they are very clear about the type of strain to include on them.

Hope it helps,

If you are measuring tumor size, particularly be optical means, it may be best to use a newly available nude NSG mouse (Jax Labs). This will give you the lack of immune response against your tumor, yet not have hair to interfere with tumor size measurements.

Any preferences for one gender over the other? My PDX are from human pancreatic cancer tissue. Would the sex of the recipient NSG mouse matter?

Thanks for your answers.

Males are usually cheaper. If there is any question about possible hormone responsiveness, then you should do some pilot studies to test if mouse gender affects your results.