i have carried out ELISA with six different conditions- three treatments with different concentrations and three controls. have already calculated the standard curve and concentration s of experimental samples. Now what's the next step i should do. which statistical tool to be used. first 24 hours i kept cells with ketones and then i added stimulant and again kept for 24 hours and checked for cytokine secretion level.
For the standard curve, the best is a logistic fit. For the data points (replicates), calculate mean +/- SEM. For comparing individual samples, use a statistical model appropriate for your samples. That depends on the question you want to answer and what kind of samples you have. Follow the Wizard of your preferred statistical tool or better talk to a statistician.
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