Dear Saleha Hafeez,

Use of a specific stain are always recommended after considering the sample conditions and objective of the research being conducted.

Here are some stains that can be used to stain red blood cells (RBCs):

1. Wright-Giemsa stain- A differential stain that is commonly used to stain blood smears. It stains RBCs a pinkish-red color, and stains the nucleus and cytoplasm of white blood cells various shades of purple

2. Romanowsky stains- A mixture of anionic eosin Y and cationic methylene blue and azure B that is traditionally used to produce hues in blood cells.

3. Chromotrope RR- A stain that can be used to stain erythrocytes red on a colorless background.

4. TrueBlack- A substance that can be used to suppress red blood cell autofluorescence for immunocytochemistry.

Discuss with your research mentor and then decide which one would be most suitable for your objective.

Accordingly use to stain red blood cells (RBCs)

Best

Shail

Do you mean counterstain to specific IHC-signal?

Or do you ask for a IHC-method?

Gudrun Lang Shail K Chaube

I have to prove the coating of RBCs with anti-RBC protein. The protein was produced in E coli and has his-tag at C-terminal. I have to prove it microscopically and for this I need specific stain or dye that can detect the complexes of antigen-antibody through ICC immunocytochemistry.

Of which species is the antibody?

recombinant antibody against the protein? reactivity of the fc-fragment?

You have to find a labelled antibody, that binds to your primary antibody.

If it has a peroxidase-label, you can make it visible via DAB-developement.

Gudrun Lang

We have produced anti-A scFv against blood type A antigen A (trisaccharide) from E. coli. We have 6X his-tag at C-terminal. We have primary anti-His antibody and secondary HRP conjugated.