Keziah Souza

2 Questions 3 Answers 0 Followers

Questions related from Keziah Souza

Facing streaking issue in native PAGE, could it be impurities?

So I have been running native PAGE (15%) for my DNA complexes, from what I observe, there is only one DNA complex as shown in image, 'RQ' which forms partially ds DNA always has some streaking...

27 May 2025 7,854 4 View

Which stain can be used for visualizing qunencher-tagged DNA oligonucleotide using gel electrophoresis?

I am trying to see a quencher (BHQ)tagged synthetic DNA oligonucleotide on an agarose gel...I used EtBr for the same...I was able to visualize the strands that I have (not tagged by BHQ), but I...

21 December 2023 2,171 0 View