Any body know Journal with high acceptance ratio?

Hello every one i need journal Names entomology related molecular study with high acceptance ratio and more then 3 impact factor, both open access and free. As well as quick one which take maximum...

09 October 2017 2,857 5 View

Primers for qRT-PCR and RNAi?

31 December 2016 7,364 8 View

I need some information please guide me from many days cant get DNA precipitation what is best for me?

use of glycogen is that help me? if yes how much volume i use for precipitation of genomic DNA for 100ul?

07 August 2016 1,143 3 View

How to make concentration high of DNA extracting from PCR Product?

Hello every one i am making dsRNA for that i need to extract DNA from PCR Product any body guide me how to get high concentration of DNA/ul because we need this DNA in as template if concentration...

06 July 2016 461 7 View

Making dsRNA using Ribomax large Scale RNA Production system how to prepare dsRNA?

03 April 2016 9,321 1 View

Anyone guide me about flanking PCR?

10 November 2015 7,314 3 View

What did mean expression of cuticle protein genes??

whats the meaning of differential expression of cuticle protein gene and there role in melanism any body can guide me or give me some related articles.??

09 October 2015 7,090 1 View

I want to know about these bands of dsRNA?

01 January 1970 385 4 View

Need help for precipitation of DNA with ethanol?

01 January 1970 2,839 10 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View