10 Questions 7 Answers 0 Followers
Questions related from Saad Jan
Hello every one i need journal Names entomology related molecular study with high acceptance ratio and more then 3 impact factor, both open access and free. As well as quick one which take maximum...
10 October 2017 2,954 5 View
Hello every one i want to know about primers for qRT-PCR and RNAi primers. I make dsRNA from ORF region of gene sequence and inject into pest at different dose. Now my question is that whether i...
01 January 2017 7,412 8 View
use of glycogen is that help me? if yes how much volume i use for precipitation of genomic DNA for 100ul?
08 August 2016 1,200 3 View
Hello every one i am making dsRNA for that i need to extract DNA from PCR Product any body guide me how to get high concentration of DNA/ul because we need this DNA in as template if concentration...
07 July 2016 563 7 View
Hello every one i need help i wana make dsRNA using Ribomax large Scale RNA Production system to use T7 promoter primer to extract DNA from PCR product but i cant understand protocol provide by...
04 April 2016 9,365 1 View
I get bands but unfortunately when I do PCR again to cut the bands and clone, I can't get bands again. I tried too many ways like different cycles, from 30---40. Also checked different...
11 November 2015 7,372 3 View
whats the meaning of differential expression of cuticle protein gene and there role in melanism any body can guide me or give me some related articles.??
10 October 2015 7,144 1 View
I have clone genes now i am making dsRNA to inject the specific gene to insect body for gene expression. Now i have Plasmid of all genes i run simple PCR with that plasmid use as template after...
01 January 1970 2,902 10 View
Hello every one i want to know weather these bands i get for dsRNA is OK or not? my dsRNA concentration is about 2500-9500 each gene but bands. i have doubt need some kind suggestion also want to...
01 January 1970 443 4 View
Hello every one i need some information i am going to express cuticle protein gene in insect body can any body guide me after how many days of fifth in star i inject dsRNA to insect body and which...
01 January 1970 7,540 0 View