For the extraction of organic compounds associated with airborne particulate matter, we used different organic solvents as Benzene, toluene, hexane, dichloro methane etc. Which solvent is suited best for the exaction of organic components associated with particulate matter ?
Hi Vishnu,
I hope you have already extracted the WSOC (water soluble organic carbon) part or polar part as Satoshi states above. You will find a number of sequential extraction procedures for particulates collected on filter...just google or consult standard literature.
For example: Rinse with hexane and acetone to separate organosulfate groups from bi-sulphate and carbonate (Russell et al., JOURNAL OF GEOPHYSICAL RESEARCH, VOL. 114, D00F05, doi:10.1029/2008JD011275, 2009)
Another one from Russell www.pnas.org/cgi/doi/10.1073/pnas.0908905107
Hi Vishnu,
we analyze IPA from particulate matter and we use hexane and acetone.
Depends on what you are interested in. Do you want to distinguish differences in effects of compounds on a biological system and determine relative toxicity. If so I would use a solvent that extracts most of the compounds and that would be a mixture of Acetone/Hexane 1:1 ratio which extracts polar and nonpolar compounds. This is also used to determine responses of cells to exposure of compounds adsorbed to PM. However, if you know something about your extract and would like to do something more specific you could do a serial extraction first with hexane alone and then Acetone. Then you can evaluate what ever response or even further fractionation with HPLC (or other methods). There are a couple of other strategies but again it depends on the question you ask.
To extract polar compounds in extract it is best to use water, ethanol methanol or butanol while for non polar compounds diethyl ether, hexane or chloroform depending on the use of the estract. I believe these will give you good result.
Polar: water, methanol, ethanol
Non-polar: hexane
For polar: 70% Methanol in water
Mid-polar: Ethyl acetate
Non-polar: Use hexane or pentane
The compounds then be separated by several analytical techniques. you can also read my papers for identification of organic compounds.
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