Which solvent can be used to dissolve standard betanin pigment and to run TLC?

04 February 2024 0 8K Report

Although standard betanin pigment is soluble in water, it is getting spread on a TLC sheet. So what can be done in this case?

Badges
Science topic

More Sakshi Thakur's questions See All

Is space-time dilation conceptually equivalent to space-time expansion?

Relativistic space-time is described as a four-dimensional continuum comprising three dimensions of space and one dimension of time. In this framework, space and time are interwoven, forming an...

29 July 2024 5,365 2 View

Does anybody know if amino acids like GABA, BABA, proline, lysine, valine or any other non-aromatic amino acid are destroyed by UV light?

I am trying to eliminate infection from my plates and therefore, gave UV treatment after addition of amino acid (used for better growth). Can you please suggest if UV destroys amino acids or not,...

02 July 2024 2,922 1 View

Why can't a black hole engulf the entire universe? What limits its gravitational reach?

Since a singularity has properties that reach infinity, one might assume its gravitational influence is also infinite. When a star collapses into a black hole, its mass is compressed into an...

27 June 2024 5,634 5 View

My material is AlSi10Mg made by Selective laser melting. Why i am facing difficulty in EBSD?

1. Which phase should I select during EBSD? 2. Attached image is the polished surface but in your opinion what is on the surface of sample? it is oxide or colloidal? 3. Diamond polishing by paste...

27 June 2024 5,248 2 View

I anyone working on synperonic F108 non ionic surfactant?

I have having issue with my DLS results

25 June 2024 2,218 1 View

Why I am facing difficulty in EBSD of AlSi10Mg (made by selective laser melting)?

I had polished my sample till 3000 grade manually then done diamond polishing by 3 micron, 1 micron and 1/4 micron (By Diamond paste and aerosol). After that I had done colloidal polishing by 0.04...

19 June 2024 5,343 3 View

Challenges in Stabilizing and Concentrating a Highly Unstable Acetyltransferase for Crystallization?

I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...

19 June 2024 2,590 0 View

Why my in vitro transcription doesn't work?

Hi everyone! I am currently doing In vitro transcription of two different RNAs, one is ~90bp size and another one ~200bp. I am using 2μg of DNA template, the reaction is kept for 3 hours at 37...

09 June 2024 4,699 5 View

Significance off zeta potential ?

Hii experts , i have synthesized a derivativ of biopolmer and zeta potential is a -1.2 mv,,can u tell me how it help me out to study my synthesized product ?

05 June 2024 4,720 3 View

How to get pure genomic DNA from CTAB method?

Hi, I have been trying to extract Plant genomic DNA via CTAB method and I am getting a 260/280 ratio ranging between 1.3 to 1.6. Any idea how to modify the protocol for fabaceae family plant...

03 June 2024 7,722 6 View

Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,793 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

How can I measure the thickness of thin film by Uv-Vis?

06 August 2024 7,810 3 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View