Which software is best for Degenerate primers designing?

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Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

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28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Treating Intermediate Variable in Linear Regression Analysis in SPSS?

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25 February 2021 4,591 5 View

Modeling viscosity using model of Chung?

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25 February 2021 8,053 5 View

RNA-seq DEGs downstream analysis and reporting in literature?

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23 February 2021 8,969 1 View

How can we study the adsorption efficiency of microgel at the oil-water interface?

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19 February 2021 2,787 1 View

Why at farmer level Cow/Buffalo are not done artificial insemination just after 2 month for yearly calving?

Physiologically in cow, oestrous heat again goes on the peak after 2 months but locally this is not observed and AI is after two months but done after at least 6 months.

17 February 2021 685 4 View

RDF calculation of water inside the Carbon nanotubes?

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17 February 2021 1,066 3 View

Is there any solar electric power plant having the concentrated photovoltaic modules?

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16 February 2021 3,796 9 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

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03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

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02 March 2021 4,029 3 View

How to calculate BREQ 3 questionnaire?

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02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

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02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

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02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

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02 March 2021 2,300 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Muhammad Awais Zahid

You can try at these two places

http://primer3.ut.ee/

bibiserv.techfak.uni-bielefeld.de/genefisher2/

Zia ur Rehman

You can use these Primer(http://www.premierbiosoft.com/),

FastPCR (http://www.biocenter.helsinki.fi/bi/Programs/

fastpcr.htm) or ORFprimer (http://www.proteinstrukturfabrik.

de/ORFprimer).