Which simple sequence repeats (SSR) primers -with high polymorphic power - can I use to analysing date palm caltivars?

More Walid Khalilia's questions See All

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,843 7 View

Would you please send me the license key for SmartPLS 04 software? I will be very thankful for this act of kindness..?

my email : [email protected]

13 April 2024 3,830 1 View

Entropy and time?

Which one dominate the other enrtopy or time?

25 January 2024 1,637 1 View

If time stops what happens to enrtopy?

let assume we are in spacecraft travelling in speed of light , droplet of ink drops in a cup of water ..theoritcally Time stops ! what is the behaviour of the droplet when it hits the water ?

24 January 2024 5,437 5 View

How calculte the force of a Car hits a wall ?

How to calculte the collision of a car with a wall with steady velocity (acceleration is 0)

24 January 2024 1,164 1 View

I want to fit Havriliak–Negami function for real and imaginary dielectric value in MATLAB or OriginPro. could help suggest me the build up equation?

I want to fit the Havriliak-Negami function for real and imaginary dielectric values in MATLAB or Origin software. Can anyone suggest the build-up equation? and how do I put initial guesses?

10 January 2024 5,467 1 View

Asking for research proposal ?

can you propose me a subject to procede my research ? i'am mechanical enginner holding master's degree in ENERGETICS

20 November 2023 6,068 2 View

Asking for research proposal ?

can you propose me a subject to procede my research ? i'am mechanical enginner holding master's degree in ENERGETICS

19 November 2023 6,461 2 View

Reduction to the Pole in the region of Notable Magnetic Field Parameter Fluctuations?

Dear colleagues, How to perform the Reduction to the Pole for a large study area where variations in magnetic field parameters (inclination and declination) exhibit significant changes? For...

17 August 2023 8,132 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

How do microbial communities contribute to ecological cycles on Earth and how does an agroecological system support diverse microbial communities?

25 July 2024 5,197 2 View