Hello!
Small liposomes (100-200 nm) can hardly be pelleted using a centrifuge. I want to prepare saccharide-loaded liposomes, which could be pelleted relatively easily (I plan to make a protein-liposome binding assay).
I came across two protocols that describe loading of liposomes with a saccharide and subsequent centrifugation:
loading with 250 mM raffinose pentahydrate (described in 10.1007/978-1-62703-401-2_24 )
and
loading with 200-400 mM trehalose (described in 10.1016/j.cryobiol.2007.06.003)
Is there a rational way to choose the most appropriate saccharide and its concentration?
Linna Green
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