Can I stabilize the ninhydrin reagent without nitrogen purge?

I am trying to use the ninhydrin assay method to determine hydrolysis degree of whey proteins without nitrogen atmosphere. I used ninhydrin reagent in the ethylene glycol - sodium acetate solvent...

03 January 2021 6,976 2 View

Presence of ZnAl2O4 by XPS?

I have a coating with 2 different layers (Al and Zn). After a process under temperature, I want to evaluate the presence of ZnAl2O4 among other oxides (mainly Al2O3 and ZnO). Is possible to...

16 June 2019 9,675 3 View

Can you use DTT in a qPCR reaction?

Is it possible to add a DNA sample containing 2 mM DTT in a qPCR reaction with detection with SYBR greenER. Samples are diluted 10x in the PCR master mix which means that the end concentration is...

13 April 2017 5,394 2 View

How can I calculate area per particle ?

02 February 2017 5,444 3 View

How can I clean the silver particles after green synthesis to SEM and TEM?

17 November 2016 477 8 View

How can I measure the concentration of C60 fullerene (nanoparticles) in tissues?

I have exposed clams in vivo to C60 fullerene for 7 days. And I would like to measure a possible accumulation in gills and digestive gland. If you can help me. Thanks in advance.

17 December 2014 5,369 6 View

Can anyone tell me how I can separate anthraquinones?

I`ve been trying separate anthraquinones with no success.

09 January 2014 9,566 3 View

Can anyone tell me if the bacteria inoculated by i.p. are eliminated with urine or feces?

I need to perform a murine sepsis model with P. multocida and M. haemolytica. But for this, I need to know if these bacteria could be eliminated by urine or feces in order to avoid contamination...

04 October 2013 2,285 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View