I am looking for an alternative to TaqMan™ Universal PCR Master Mix, chepar than it if possible. It have to be compatible to be run in many different qPCR devices, it shouldn't be optimized for a specific qPCR device.
Roche Eagle Taq can be an option, imo.
What are your suggestions?
Hi Oguz, We use SYBR green by Thermo Fisher on an Eppendorf RealPlex, even if it is not amognst the compatible instruments it works fine, SYBR should work everywhere and the price is acceptable (In italy, at least).
http://www.thermofisher.com/it/en/home/life-science/pcr/real-time-pcr/real-time-pcr-reagents/sybr-green-real-time-master-mixes.html?gclid=Cj0KCQjwwLHLBRDEARIsAN1A1Q7zK0DF8WlwsDYpug3Hp3O512Ky1faPhC7_kkboIpHeGQCgHbuf8SUaAsf7EALw_wcB&s_kwcid=AL!3652!3!77558507892!e!!g!!sybr%20green&ef_id=Vy@CSQAABAJOeDe9:20170717200258:s
Hi Oguz, try our SensiFAST (http://www.bioline.com/sensifast#probe), if you speak to our distributor (www.ardimed.com) you will be able to get a sample to test.
Dear Anne
Did you try to open the experiment in QuantStudio and change the used reagent in experiment properties and/or samples assignment?, I guess the problem here is a TaqMan mastermix being processed by the instrument as SYBR and vice versa
watch this video dealing with experiment setup fixes in StepOne instruments (pretty much the same as QuantStudio)
https://www.youtube.com/watch?v=gYTR7HgWNNo
good luck
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