How to select better housekeeping gene in wheat ?

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Is binary annotation is enough for object detection deep learning. Or should we seperate each type object to different classes?

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Cytokine stimulation from mouse spleen cells with LPS ?

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Making triplicates for repeat samples rather than duplicates in qPCR ?

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Missing citation number?

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Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

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Why my colony PCR results of my recombinant bacterial not showing any results?

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What is the acceptable p-value cutoff for GO enrichment analysis ?

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Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

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Hello Researchers, Can anyone guide me on estimating the Leaf Area Index (LAI) of wheat using only weather and soil parameters? Is it possible?

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Could GSH level be more in CCL4 rats than negative control rats?

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If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

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25 July 2024 4,149 2 View

Christophe Hano

Two interesting papers on the reference gene validation:

https://pubmed.ncbi.nlm.nih.gov/18433420/

http://www.plantcell.org/content/20/7/1734

More specifically for wheat,

There are some nice published papers on the subject:

For example for abiotic stress

Article Identification of stable reference genes for qPCR studies in...

Developmental process

https://www.nature.com/articles/s41598-020-59580-5

With kind regards

Christophe

Muhammet Cagri Oguz

Mr. Christophe, thank you for your reply. Best regard