Making triplicates for repeat samples rather than duplicates in qPCR ?

Hi, I have been doing Reverse Transcriptase qPCR on some liver mRNA samples. I am targeting liver inflammation genes like Col1a1, alpha SMA, IL6, IL1B and etc... Even though mRNA concentrations...

17 December 2020 6,165 3 View

Missing citation number?

there is no citation number at one of my papers (its file is attached), but in "google scholar" this paper has 7 citation number, in "publons" it has 5 .

15 June 2020 5,641 7 View

Should gene expression determined by RNA-Seq be controlled by Real-Time PCR?

Is it necessary to perform RT-PCR analysis of genes that show an increase or decrease? Or is it just for control purposes in RNA-Seq Analysis?

19 February 2020 207 5 View

How to calculate electric poling parameters ( voltage, electric field, etc) for PVDF and PVDF-TrFE thin film piezoelectric samples?

I'm trying to find parameters for electric poling to increase the output voltage of piezoelectric nanogenerators.

15 July 2019 2,769 7 View

How do molecules(spesifically proteins and RNAs) knows which way to advance to find and interact with the molecules they should be interacted?

Title explains itself. I am trying to find information about a question in my mind. For example if we divide a cell by regions like west east noth south, a tRNA going out to look for its spesific...

23 February 2019 1,074 7 View

Is there any big difference between AMOS and LISREL in SEM analysis? Which one is better?

Is there any big difference between AMOS and LISREL in SEM analysis? Which one is better?

15 June 2018 5,269 15 View

Some questions about Crispr/Cas 9 (T7 Assay, extra band expectation, positive control, alternative method to single cell isolation and expansion ?

We have psp cas 9 2a puro and esp cas 9 1.1 vectors. We are performing T7 assays for mutation detection. Do we have the possibility to use positive control? Do we have the chance to estimate how...

13 May 2018 5,372 4 View

Why does agarose gel look smear image and background composition ?

04 June 2017 3,029 2 View

How reliable are contigs?

Hi, Im learning about next gen. seq. So, with NGS you shear up dna into fragments and when the computer tries to align by matching overlapping regions isnt there a possibility of the fragments...

08 January 2017 154 4 View

Which concentration of rifampin and kanamycin can I use to prepare Agrobacterium growing media?

19 October 2016 872 6 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View