Carpomyia vesuviana Costa is monophagous, destructive pests, and endogenous species infesting on Zizyphus spp.

I have tried many primers with gradient PCR for their molecular identification but they and not working properly.

Primers Names

X-F: 5′-ACG ATG ATG CGA TTG GTG AC-3 ′

X-R: 5′-TAT TGG TCG CGG AAC AAA CC-3 ′

COI CLepFolF 5'---ATT CAA CCA ATC ATAAAG ATA TTG G

CLepFolR 5'--TAA ACT TCT GGA TGT CCA AAA AAT CA

LCO1490 and HCO2198