Primers used by Joycee et al, 1994 are good enough to produce ITS regions. But now days multigene sequence analysis is used for identification of nematodes. You can also follow the protocol as suggested by Vrain et al.
Dear Houda Kawas, mainly following regions of taxonomic importance are used for identification nematodes
Primer Sequence 5'–3' Reference
TW81 (ITS) GTTTCCGTAGGTGAACCTGC Joyce et al. (1994)
AB28 (ITS) ATATGCTTAAGTTCAGCGGGT Joyce et al. (1994)
5.8SM2 CTTATCGGTGGATCACTCGG Zheng et al. (2000)
5.8SM5 GGCGCAATGTGCATTCGA Zheng et al. (2000)
D2A (28S) ACAAGTACCGTGAGGGAAAGTTG De Ley et al. (1999)
D3B (28S) TCGGAAGGAACCAGCTACTA De Ley et al. (1999)
The above primers can be helpful in identification of H. schacthi.
Dear Aashaq Hussain Bhat
Thanks for your efforts.
Best regards
Houda Kawas
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