I am detecting mutations in virus genome, using PCR for 1kb followed by Sanger sequencing. GoTaq (Promega) works greatlly, but PCR with Pfu Polymerase gives a small amount of amplicon. I followed all the instructions of elongation time, anneling temperature ...
Which enzyme should I use instead of Pfu Polymerase, that is a high fidelity (and not sooo expensive)??