Which Polymerase high fidelity is best to detect mutations (PCR followed by Sanger sequencing)??

I am detecting mutations in virus genome, using PCR for 1kb followed by Sanger sequencing. GoTaq (Promega) works greatlly, but PCR with Pfu Polymerase gives a small amount of amplicon. I followed...

13 March 2018 4,677 4 View