Supercoiled dna will form a small sphere and run more quickly than linear dna which is long and has more solvation effects on its surface so linear will run slower in agarose gels

Supercoiled will run the fastest followed by the circular plasmid. Restriction digested plasmid will have the least mobility.

Hi Pudi,

When uncleaved plasmid DNA is isolated and run on an agarose gel, two, three, and even four or more bands may be observed. Hopefully most of your isolated DNA will be supercoiled, but there may be other forms as well. In the attachment I am sending an example pattern of bands from agarose electrophoresis.

Best regards