At first, you have to use "peaks deconvolution", after that, calculate area of each peak

Good luck

The number of acid sites is the amount of ammonia desorbed (provided that you flushed off the physiosorbed ammonia prior to your TPD-step). The number of acid sites in mmol/g you obtain by dividing the volume of ammonia adsorbed in ml(STP)/g by the molar volume of an ideal gas (22.414 ml/(STP)/mmol).

The strength of the acid sites you obtain by evaluating the activation energy for desorption (although this is typically done by simply looking at the peak temperature maximum - in the absence of diffusion limitations the activation energy for desorption in kJ/mol is the peak maximum temperature in K divided by 4)

thank you Le Phuc Nguyen and Eric Van Steen for your valuable suggestions

multiple the amount of ml/g with 44.614 then you will get the acidity in micromol/gram.