Arseniy A. Lobov

3 Questions 9 Answers 0 Followers

Questions related from Arseniy A. Lobov

Does anyone know a good backbone for 15-40 amino acid peptides overexpression by lentiviral transduction in mammalian cells?

I want to check the effect of peptide overexpression on mammalian cells, but I have failed to find a suitable lentiviral vector. Ideally, I would like an inducible expression with a puromycin...

24 March 2025 2,654 0 View

Should I expect for cross-linked band to have expected MW on WB?

I am doing western blotting for sample after PFA cross-linking. And, indeed, I see something which looks like cross-linked band after PFA, but it MW is ~30 kDa smaller than expected. I would like...

09 January 2024 9,624 3 View

Which one tag is better for pull-down assay - GST of His-tag?

We are planning to perform pull-down assay, but I am a new in that field. Which tag for affinity purification of the “bait ” protein is better? is there really significant differences between GST...

22 June 2022 1,926 2 View