Intégration-free reprogramming methods generate iPSCs that do not contain detectable vectors or transgenes. Traditional technologies used for reprogramming (e.g., lentivirus, retrovirus) integrate into the genome of the target cells. The resulting iPSCs and cells differentiated from those iPSCs will contain foreign DNA and could be unsafe and problematic for use in cell therapy and drug discovery applications. Furthermore, the integration could occur in a critical region of the genome, causing problems with unrelated developmental processes.
I've never attempted bovine reprogramming, but one "traditional technology" is the use of Sendai virus, which does not integrate into the genome.
Of course, RNA reprogramming has become much more robust as well, so that is another consideration for non-integrating reprogramming.
Hi Jam,
Yes sendai vectors is one way, the problem is that you have to find the good balance bteween efficiency/toxicity because this type of vector can be very toxic.
Another way is to use non integrating lentiviral vectors or MLV, in this case you have no problem of toxicity but you have to use very efficient vectors to obtain good functional results.
Regards
Nicolas
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