Actually, I want to know which method should be used to collect blood from rats because the jugular vein has to be exposed to collect blood which make rat experience a small trauma and additionally there may be a chance of getting infection on that exposed site.
Alternatively retro orbital is very very painful for rats.
Retroorbital is indeed painful and should be avoided whenever possible, since rats are our friends!
...what about the tail vein (especially for small volumes an repeated sampling)? The tail can easily be disinfected to avoid infection.
Tail vain is good way for collecting small amounts of blood for repeated sampling! You just need to keep its tail in warm water for like 1-2 min and after that everything is easy and standard for blood colection :)
Depending on amount of blood you need.
I f you need small amount of blood tail vein is the best one but if you need large amount femoral vein is good
but the largest amount of blood you collect from heart puncture but need more experience you can collect without dying the rats
I will suggest tail vein. Just keep it warm for a little bit (by hand or in warm water). You can use this route for small amounts of blood.
Good luck!
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