Meyer et al. and Chubukov et al. propose 60 % cold ethanol (-20°C) followed by water extraction for gram-positive Bacillus subtilis and Staphyloccocus aureus:
http://www.microbialcellfactories.com/content/12/1/69
http://www.sciencedirect.com/science/article/pii/S0003269710001557
http://msb.embopress.org/content/9/1/709
Bolten et al. used boiling water (100°C) for extraction from different bacteria and yeast:
http://pubs.acs.org/doi/abs/10.1021/ac0623888
http://link.springer.com/article/10.1007%2Fs10529-008-9789-z
For gram-negative Escherichia coli, Maharjan and Ferenci suggest 50 % cold methanol (-40°C):
http://www.sciencedirect.com/science/article/pii/S0003269702005365
I hope that helps.
M
hey Michael.. thanku so much for ur help.. i want to extract intracellular metabolites from Lactococcus lactis... i will try protocol which is there for gram positive bacteria... will come back with the results..
Most of the time 70-80 % ethanol (chilled) works, i think it should work
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