Meyer et al. and Chubukov et al. propose 60 % cold ethanol (-20°C) followed by water extraction for gram-positive Bacillus subtilis and Staphyloccocus aureus:
hey Michael.. thanku so much for ur help.. i want to extract intracellular metabolites from Lactococcus lactis... i will try protocol which is there for gram positive bacteria... will come back with the results..