.
In the case of E coli, the proportion of NAD+ to NADH could be about 7 (or 8) to 1 (umol/g of dry cell weight).
I am looking for a protocol for ion pair reverse phase hplc using Luna C18(2) column for estimation of intracellular nucleotide precursors (e.g. UTP, UDP, ATP, AMP etc.). The major concern is that...
02 March 2014 5,319 5 View
Which is more efficient in the case of bacterial system?
02 March 2014 4,722 3 View
I want to quantify UDP-Glucuronic acid and UDP-Nacetylglucosamine in my bacterial samples using Ion Pair reverse phase HPLC. The paper I am referring for the protocol says to look for their peeks...
02 March 2014 741 0 View
I need to add reactions to an already existing set of reactions used for metabolic flux analysis for a pathway. While doing that I am getting all random and unrealistic values for fluxes. What...
31 December 2013 7,203 5 View
I know one kit but I am looking preferentially for some colorimetric analysis or quantification using fluorescence spectroscopy. Help regarding any of the methods would be appreciated.
31 December 2013 9,033 5 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View