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In the case of E coli, the proportion of NAD+ to NADH could be about 7 (or 8) to 1 (umol/g of dry cell weight).
I am looking for a protocol for ion pair reverse phase hplc using Luna C18(2) column for estimation of intracellular nucleotide precursors (e.g. UTP, UDP, ATP, AMP etc.). The major concern is that...
02 March 2014 5,220 5 View
I want to quantify UDP-Glucuronic acid and UDP-Nacetylglucosamine in my bacterial samples using Ion Pair reverse phase HPLC. The paper I am referring for the protocol says to look for their peeks...
02 March 2014 639 0 View
Which is more efficient in the case of bacterial system?
02 March 2014 4,617 3 View
I need to add reactions to an already existing set of reactions used for metabolic flux analysis for a pathway. While doing that I am getting all random and unrealistic values for fluxes. What...
31 December 2013 7,100 5 View
I know one kit but I am looking preferentially for some colorimetric analysis or quantification using fluorescence spectroscopy. Help regarding any of the methods would be appreciated.
31 December 2013 8,944 5 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...
28 February 2021 4,851 8 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...
25 February 2021 1,675 3 View