Mandeep Kaur

6 Questions 12 Answers 0 Followers

Questions related from Mandeep Kaur

Which one is better for extraction of intracellular metabolites: absolute ethanol or 70% ethanol?

Which is more efficient in the case of bacterial system?

03 March 2014 4,671 3 View

Can anyone recommend a protocol for ion pair reverse phase hplc?

I am looking for a protocol for ion pair reverse phase hplc using Luna C18(2) column for estimation of intracellular nucleotide precursors (e.g. UTP, UDP, ATP, AMP etc.). The major concern is that...

03 March 2014 5,280 5 View

Can anyone suggest any other wavelength that can be used for UDP-Glucuronic acid and UDP-Nacetylglucosamine estimation with minimum noise?

I want to quantify UDP-Glucuronic acid and UDP-Nacetylglucosamine in my bacterial samples using Ion Pair reverse phase HPLC. The paper I am referring for the protocol says to look for their peeks...

03 March 2014 699 0 View

Adding reactions to already existing mfa model - can anyone help?

I need to add reactions to an already existing set of reactions used for metabolic flux analysis for a pathway. While doing that I am getting all random and unrealistic values for fluxes. What...

01 January 2014 7,162 5 View

Is there any method for quantification/estimation of NAD/NADH using colorimetric method?

I know one kit but I am looking preferentially for some colorimetric analysis or quantification using fluorescence spectroscopy. Help regarding any of the methods would be appreciated.

01 January 2014 8,996 5 View

What is the approximate range of concentration of NAD/NADH in bacterial samples?

01 January 2014 9,236 1 View