How does the gelling medium play a role in supply of nutrients to the ex-plant?
The only thing I heard is that with Phytagel one can have problem with Kanamycin selection. But I have no personal experience with it.
Phyta gel is better as we have seen the results in rice plantlet regeneration
Phyta gel is better as more clear/transparent media preparation is possible
It depends on the specific application. Both of them have advantages and disadvantages. This is from Plant Cell Culture Protocols, Methods in Molecular Biology vol. 111:
Agars and agaroses generally produce gels that are stable for prolonged periods and are considered not to bind media components excessively. Products with various degrees of purity are available, and low-gelling temperatures types can even enable embedding of sensitive cells, such as protoplasts. On the other hand, Gelrite/phytagel produces a rigid gel at much lower concentrations than agar or agarose. They are also almost transparent, which makes it easier, e.g., to identify contamination at an early stage. These gels do, however, tend to liquify in long-term cultures owing to pH changes or the depletion of salts necessary for crosslinking. Higher concentrations of antibiotics (e.g., kanamycin) may also be required in Phytagel/gelrite solidified media in comparison to those solidified with agar/agarose.
I have used both before in wheat and barley. Phytagel works much better than agar-agar.
In invitro screening for Striga resistance, can I use phytagel instead of agar?
it spends on the superpose of your experiment. Agar is known as the best gelling agent, however in some cases phytagel used for its transparency and cheaper price.
Gustavo Acevedo-Hernandez : could you please let me know the full citation details of this interesting article in "Plant Cell Culture Protocols, Methods in Molecular Biology vol. 111:"
As respostas irão depender da espécie vegetal utilizada. O Phytagel é mai nítido e mais barato. Porém a escolha dos gelificantes vai depender de cada situação pois a informações na literatura que o Phytagel é melhor e em outras o Agar-agar
Agar has long been used to solidify media for PTC. The type of agar or gelling agent used can influence the growth of the tissue in culture. Both purity and cost of the gelling agent are important factors in any research or production operation.
Both Phytagel and Agar are great. Your choice is related to a specific application, such as the micropropagation phase, or even the species of plant you are working on. This means that the chosen gelling agent can make a difference.
Agar is definitely the best and most widely used culture medium. Phytagel is a very solidity medium and is only suitable for detecting contamination.
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