Which one better to start extraction with methanol first to obtain the crude extract, the we dissolve it in water and do partition it with , CHCl3 , ethyl acetate, PE and latter hexane or start partition with Hexane then PE then Ethyl acetate, and later CHCl3. ?
You have previously carried out an extraction using methanol. At this point, you're inquiring about the ideal solvent sequence for the fractionation process.
The solvents you mentioned exhibit varying levels of polarity, with n-hexane being the least polar, followed by petroleum ether (PE), then chloroform, and finally ethyl acetate being the most polar.
To proceed for the fractionation procedure, begin by dissolving your methanolic extract in water.
Now, start by fractionating with n-hexane, then PE, followed by chloroform, and finally ethyl acetate.
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