Which of the currently existing dsRNAi vectors works best in Drosophila testis/sperm?

10 August 2013 1 3K Report

NIG, VDRC or the TRiP (V19,V20,V22) collections is most efficient?

I would recommend using the new TRiP lines, the combination of site specific insertion with gypsy insulator sequences to ensure high transcription makes for reliable lines, which are easy to obtain from the BDSC rapidly and at a low cost per line.

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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