How exelent manner use for prevent shrink the tissue when use SEM procuder?

When i exam under SEM the cell is shrinks

31 December 2018 5,985 6 View

When I do blaked by osmium and i do its procedure to rach hydration by alchohool and want to save this tissue which sutable manner?

i save it in absolute alcohol or 70 per. And which period can save it befor examing by SEM.

31 December 2018 8,120 2 View

The particle such as desmosome can be apear under SEM at1micrometer? I mean inegrin protein that apear tiny bal or non ?

I exam the surface of the cell and found tiny ball above fibers of basal lamina of epithelial cell

09 October 2018 7,276 2 View

I need extract the EGF wich optimal speed of ultracenterfuge?

For extract EGF from rat

09 October 2018 9,281 2 View

The myoepithelial cells have many shape each shape have special localization in salivary gland?

This question important for describ the tissue .also the salivary gland mapping emagenation .

08 September 2018 4,692 2 View

Howmuch concentration of the sodium in the parotid tissue and submandibular gland?

the elecyrolyte of sodium concentration may be detected the saliva flow rate

08 September 2018 3,328 3 View

When we see a child we smile and show us the features of happiness why?

the any child that send happiness in hart any person.

01 January 1970 1,448 21 View

Some people use good ling were thinking its good.

use good or white lying that caouses many proplem in community

01 January 1970 4,204 7 View

Why so many colors and see the flowers are happy?

Always when I see colors and flowers so l am very hapy

01 January 1970 6,276 10 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View