Which new immunohistochemistry stain for myoepithelial cell ?

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When I do blaked by osmium and i do its procedure to rach hydration by alchohool and want to save this tissue which sutable manner?

i save it in absolute alcohol or 70 per. And which period can save it befor examing by SEM.

31 December 2018 8,252 2 View

How exelent manner use for prevent shrink the tissue when use SEM procuder?

When i exam under SEM the cell is shrinks

31 December 2018 6,101 6 View

The particle such as desmosome can be apear under SEM at1micrometer? I mean inegrin protein that apear tiny bal or non ?

I exam the surface of the cell and found tiny ball above fibers of basal lamina of epithelial cell

09 October 2018 7,388 2 View

I need extract the EGF wich optimal speed of ultracenterfuge?

For extract EGF from rat

09 October 2018 9,393 2 View

The myoepithelial cells have many shape each shape have special localization in salivary gland?

This question important for describ the tissue .also the salivary gland mapping emagenation .

08 September 2018 4,837 2 View

Howmuch concentration of the sodium in the parotid tissue and submandibular gland?

the elecyrolyte of sodium concentration may be detected the saliva flow rate

08 September 2018 3,441 3 View

When we see a child we smile and show us the features of happiness why?

the any child that send happiness in hart any person.

01 January 1970 1,585 21 View

Some people use good ling were thinking its good.

use good or white lying that caouses many proplem in community

01 January 1970 4,324 7 View

Why so many colors and see the flowers are happy?

Always when I see colors and flowers so l am very hapy

01 January 1970 6,404 10 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

Jaydip Datta Popular answer

Histopathological Chemical Analysis - following .

Snow Nie

https://www.creative-bioarray.com/Services/Histology-Services.htm

You can have a try, ask the scientific team.

Jasvinder Kaur Bhatia

Glial Fibrillary Acidic Protein (GFAP), S-100, Calponin, p63 in addition to Smooth muscle actin,CK5/6,

Luma HAFEDH ABED Oneizah

Good quation

Wafaa Alhashemi

Thank you dearjasvinder, are you know the number of MECS primary cytoplasmic processing study the description of MEC.

Jaydip Datta