How exelent manner use for prevent shrink the tissue when use SEM procuder?

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When I do blaked by osmium and i do its procedure to rach hydration by alchohool and want to save this tissue which sutable manner?

i save it in absolute alcohol or 70 per. And which period can save it befor examing by SEM.

31 December 2018 8,120 2 View

Which new immunohistochemistry stain for myoepithelial cell ?

need new stain not ruotin stain of immunocytochemistry suchas a MSA and keratinocyte and vimentin

09 October 2018 9,787 6 View

The particle such as desmosome can be apear under SEM at1micrometer? I mean inegrin protein that apear tiny bal or non ?

I exam the surface of the cell and found tiny ball above fibers of basal lamina of epithelial cell

09 October 2018 7,276 2 View

I need extract the EGF wich optimal speed of ultracenterfuge?

For extract EGF from rat

09 October 2018 9,281 2 View

The myoepithelial cells have many shape each shape have special localization in salivary gland?

This question important for describ the tissue .also the salivary gland mapping emagenation .

08 September 2018 4,692 2 View

Howmuch concentration of the sodium in the parotid tissue and submandibular gland?

the elecyrolyte of sodium concentration may be detected the saliva flow rate

08 September 2018 3,328 3 View

When we see a child we smile and show us the features of happiness why?

the any child that send happiness in hart any person.

01 January 1970 1,448 21 View

Some people use good ling were thinking its good.

use good or white lying that caouses many proplem in community

01 January 1970 4,204 7 View

Why so many colors and see the flowers are happy?

Always when I see colors and flowers so l am very hapy

01 January 1970 6,276 10 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

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What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

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Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

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After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

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What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

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I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

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Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

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How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

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Shall I skip the measurement model in SmartPLS?

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What is involved in morphological changes of SEM images of a substance?

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Vladimir Dusevich

To minimize shrinkage use standard procedures for biological specimen preparation for SEM

https://www.google.com/search?q=sem+biological+sample+preparation&rlz=1C1OPRB_enUS553US553&oq=sem+biolo&aqs=chrome.2.69i57j69i59l2j0l3.9662j0j7&sourceid=chrome&ie=UTF-8

Wafaa Alhashemi

Thank you but many manners that found in internate some of one washing by alcohol but som others use distal water and alcohol

https://research.jcu.edu.au/archive/enabling/aac/cairns-aac/biological-sample-preparation-for-the-sem

https://www.intechopen.com/books/modern-electron-microscopy-in-physical-and-life-sciences/sample-preparations-for-scanning-electron-microscopy-life-sciences