The myoepithelial cells have many shape each shape have special localization in salivary gland?

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How exelent manner use for prevent shrink the tissue when use SEM procuder?

When i exam under SEM the cell is shrinks

31 December 2018 5,985 6 View

When I do blaked by osmium and i do its procedure to rach hydration by alchohool and want to save this tissue which sutable manner?

i save it in absolute alcohol or 70 per. And which period can save it befor examing by SEM.

31 December 2018 8,120 2 View

Which new immunohistochemistry stain for myoepithelial cell ?

need new stain not ruotin stain of immunocytochemistry suchas a MSA and keratinocyte and vimentin

09 October 2018 9,787 6 View

The particle such as desmosome can be apear under SEM at1micrometer? I mean inegrin protein that apear tiny bal or non ?

I exam the surface of the cell and found tiny ball above fibers of basal lamina of epithelial cell

09 October 2018 7,276 2 View

I need extract the EGF wich optimal speed of ultracenterfuge?

For extract EGF from rat

09 October 2018 9,281 2 View

Howmuch concentration of the sodium in the parotid tissue and submandibular gland?

the elecyrolyte of sodium concentration may be detected the saliva flow rate

08 September 2018 3,328 3 View

When we see a child we smile and show us the features of happiness why?

the any child that send happiness in hart any person.

01 January 1970 1,448 21 View

Some people use good ling were thinking its good.

use good or white lying that caouses many proplem in community

01 January 1970 4,204 7 View

Why so many colors and see the flowers are happy?

Always when I see colors and flowers so l am very hapy

01 January 1970 6,276 10 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View