Hiii all...
I am working on neurological disorder which includes neurodegenerative as well as neurodevelopmental disorder, I am facing problem to grow IMR -32 cell lines in my lab. I have tried all suggested troubleshooting and also tried growing them with 20% FBS. Now I am looking for an alternative cell lines which can be used for various neurological disorders. I thought of taking SH-SY-5Y cell lines but these cell lines shows mixed nature i.e. may be suspension or adherent, which I feel is difficult to handle for MTT assay. Few more cell lines in my mind are LN-18, SK-N-MC and SK-N-SH. Can anyone suggest me the best cell lines which I can work with?
Thanks in advance
Dear Shubham Dwivedi,
If you are only interested in cell lines derived from human tissue, then i think SH-SY5Y will be a good alternative choice. This line was widely used in the literatures and in neurological studies especially in PD-related areas. As for the cultural conditions, based on my experiences, most of SH-SY5Y cells are adherent to the culture dishes although there are a small portion of suspension cells in the medium. But i don't think it will be a problem for your MTT assay. AND it depends on where you get the SH-SY5Y cell line, some subcolones of this line contains almost no suspension cells.
Good luck for your experiment.
Dear Yingjun Liu,
Thank you for the reply.
But I would like to add that when we do MTT assay, we are supposed to plate equal number of cells in each well, but if even few cells are in suspension they may be discarded with media during the MTT assay process which may again lead to unequal distribution of cells and ultimately false data may be generated.
I don't know how valid is my point but this may be a possibility..
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