Which MWCO Dialysis membrane is suitable for in vitro drug release of Drug loaded nanoparticles of size ~50 nm from TEM?
Dialysis membrane 70, HIMEDIA, molecular weight cut off 12 000–14 000 Daltons; pore size: 2.4 nm can be useful.....
50 nm particles are large compared to the MWCO of almost any dialysis tubing, so you should be able to use pretty much whatever tubing you like for dialyzing small molecules. Avoid very low MWCO tubing (like 2,000 Da) because of the slowness of dialysis. I would use a 20,000 MWCO dialysis cassette.
In vitro release experiment through dialysis tube mostly depends on molecular weight of your drug that is to be encapsulated or tethered with your polymer. so if the M.Wt of your drug or desired compound to whom you measured its release is less than 1000 than use can use MWCO tubing of 2000 Da...
Molecular weight of the drug I am using is 338.236 g/mol. So can i use Dialysis Membrane-60 Av. flat width - 25.27 mm, Av. diameter - 15.9 mm, capacity approx - 1.99 ml/cm.
i have similar question too... i want to buy dialysis bag for my experiment that is sustained release of dalbavancin with 1816.7 molecular weigh and i dont know i have to buy 12 kda or 14 ? please help me.
Either one will do. The particle size is undoubtedly much greater than 14 kDa, which is the size of a small protein, only a few nm in diameter. In fact, an even larger MWCO would be even better.
You may go through 12000-15000 Da pore size 2.4 nm for your drug release assay. Good luck.
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