Which mounting reagent should be used for BODIPY493/503?

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Chandni Sood

Thanks Zaffar actually most papers show the 2 which I have already written

Veijo T Salo

Hi!

All of those should work ok, but be vary that lipids may diffuse and lipid structures such as intracellular lipid droplets may fuse after fixation. To avoid such artefacts consider imaging quite soon after mounting (and imaging all samples to be compared within the same imaging session). Alternatively you could mount with PBS and seal with glue or nail polish or not mount at all (ie use dishes with glass bottoms after fixation and staining).