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I am doing silver staining using standard protocol with 0.02% Sodium Thiosulfate (2min sensitization)(Na2S2O3) ,0.2% AgNO3 (20min)and 6% Na2CO3 but i am getting dark background while developing my...
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I am trying to knockout a bacterial gene by homologous recombination. I have designed a knockout construct having 500 upstream, 500 downstream, 100 bp of each 5` and 3` end of the gene with a...
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