Chandni Sood

5 Questions 13 Answers 0 Followers

Questions related from Chandni Sood

Which mounting reagent should be used for BODIPY493/503?

I have been using Anti-fade Prolong Gold but observed diffused bodipy florescence. I came across Mowiol-488 (Calbiobchem) and Fluoromount-G (southern biotechnology) has been used in papers. Which...

22 September 2016 3,011 2 View

Why does my silver stain have a dark background ?

I am doing silver staining using standard protocol with 0.02% Sodium Thiosulfate (2min sensitization)(Na2S2O3) ,0.2% AgNO3 (20min)and 6% Na2CO3 but i am getting dark background while developing my...

08 May 2015 2,401 6 View

How can I prepare salmonella cell lysate?

I would like to check the concentration of protein which is not expresses in detectable amount in salmonella secretory soup.

03 March 2015 4,918 2 View

What should be done when a protein is getting precipitated during dialysis?

I purified my recombinant His tag protein in NaHPO4-50mM, Nacl-300mM,immidazole-20mM,bmercaptoethanol-10mM, glycerol-10% with pH 8 (pI of protein 9.45) and eluted in 500mM Imidazole conc. in...

20 July 2014 1,133 17 View

Does knocking out a gene can affect transcription of adjacent gene in bacteria?

I am trying to knockout a bacterial gene by homologous recombination. I have designed a knockout construct having 500 upstream, 500 downstream, 100 bp of each 5` and 3` end of the gene with a...

26 June 2014 3,087 11 View