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I have been using Anti-fade Prolong Gold but observed diffused bodipy florescence. I came across Mowiol-488 (Calbiobchem) and Fluoromount-G (southern biotechnology) has been used in papers. Which...
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I am doing silver staining using standard protocol with 0.02% Sodium Thiosulfate (2min sensitization)(Na2S2O3) ,0.2% AgNO3 (20min)and 6% Na2CO3 but i am getting dark background while developing my...
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I would like to check the concentration of protein which is not expresses in detectable amount in salmonella secretory soup.
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I purified my recombinant His tag protein in NaHPO4-50mM, Nacl-300mM,immidazole-20mM,bmercaptoethanol-10mM, glycerol-10% with pH 8 (pI of protein 9.45) and eluted in 500mM Imidazole conc. in...
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I am trying to knockout a bacterial gene by homologous recombination. I have designed a knockout construct having 500 upstream, 500 downstream, 100 bp of each 5` and 3` end of the gene with a...
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