I would like to assess a species hybridization hypothesis through Genetics. I am aware that SSR fits well but it could be quite expensive. I am looking for an alternative one that may be as effective as SSR or even better, but with a lower cost.
Thanks a lot in advance.
Hi,
Is your investigations on hybridizations limited to a particular species/genus?
Less expensive than SSR is not easy to find. Why not a morphological marker?
Dear Diego
Microsatellite (SSR) is comparatively cheaper than its alternative AFLP analysis. If you are working on plant to test the hypothesis of natural
hybridization you can also use the low-copy nuclear genes and to determine the maternal parent of the hybrids individuals, you can use chloroplast DNA markers.
Hi Ajay, Paulo, Sunil,
Yes, I am working on a particular genus and it seems that half the species may produce hybrids.
Actually the hypothesis is based on morphological features but there are still many doubts. Besides phylogeny is needed to understand problems on some species complexes.
I did not notice on low-copy nuclear genes. What about SNPs?
Thank you all for your replies.
SNPs are more expensive than SSR. Really, why not use morphological traits?
Hello
sequence-related amplified polymorphism (SRAP) is simple, inexpensive, and effective for producing genome-wide fragments with high reproducibility and versatility.
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