The presence of EPS - producing lactic acid bacteria using solid medium - containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions. EPS production was visualized by the presence of mucoid colonies on the discs, which was confirmed by formation of a precipitate when part of the colony was mixed with absolute alcohol.. This method proved to be effective and economical because severalstrains could be tested on the same plate, with immediate confirmation. For more details consult Https://www.scieio.br - pdf - cta - aop - cta_5362 .

Hi,

The cultured method of EPS production from lactic acid bacteria was depended on bacterial species. e.g. The dextran production by Leuconostoc mesenteriodes was need streak method at 70-150 rpm.

Sylvester Peter Antai thank you for your response. could you send me a paper with this method?

Regards,

Hi Paria

You can use streak method or spread plating method on MRS agar. I have obtained single isolated colonies of LAB producing EPS with both methods. I use 2% (w/v) of agar though.

Thanks

Mohit bibra