Why are functional gene results (nosZ, amoA) compared to 16S gene?

I am working on the effect of pH on functional genes in the nitrogen cycle (amoA, nosZ..). Some of the papers i have read present results based on the ratio between functional gene (like nosZ) and...

27 December 2020 1,625 6 View

Why would gel electrophoresis need to be run for 16 hours at 2-8 deg C? Is there an alternative protocol that takes less time or works at room temp?

I am trying to separate and analyze macromolecules (DNA) using horizontal gel electrophoresis. Protocol involves 1X TAE buffer, 10X denaturing buffer, 1X running buffer, 1% agarose gel, and 2X...

18 November 2020 667 2 View

Is it reasonable for a journal to take over 12 months before giving a response on whether they will publish your article or not?

I submitted an article for publication to one highly rated journal and got a response requiring major revisions within about 6 months. After making corrections, I resubmitted for a further review....

12 August 2020 1,986 3 View

How Does One turn 10 separately published articles into a book?

I am currently seeking to produce a book by selecting ten articles from my research profile. I search your input on strategies, tactics and skills of creating a flowing book out of articles that...

31 July 2020 7,664 4 View

What are the emerging theories of reading and interpretation in the Covid 19 Context?

It is constantly underlined that Covid 19 has produced a new normal in our world setting. What are the new ways of reading texts in the Covid 19 context? Do we have new theories for understanding...

31 July 2020 3,443 11 View

What is the relationship between Ubuntu//Botho philosophy and Gender?

The African philosophy of Ubuntu is highly praised for its understanding of one's humanity as the capacity to recognise the other. What is the link between Ubuntu and gender? Is Ubuntu gender...

31 July 2020 3,586 5 View

Can you please suggest me any latest research topic in the field of finance and stock market?

Can you please suggest me any latest research topic in the field of finance and stock market?

24 February 2020 1,202 11 View

Newton,Archimedes,Pascal-Well known in the field of research....At that time Y thy hvn't thought of pursuing M.Phill or P.hD. first n research later?

Newton,Archimedes,Pascal-Well known in the field of research....At that time Y thy hvn't thought of pursuing M.Phill or P.hD. first n research later? Which should come first? Higher degree or...

21 August 2019 6,087 2 View

How can l apply GA in matlab to do demand side management for an industrial plant?

I need assistance on how to apply GA in energy optimization for an industrial plant. My approach is demand side management for an industrial plant using GA in matlab. May you help with algorithms...

05 April 2019 9,372 3 View

Which antibody can I use to stain for epidermal Langerhans cells in rat skin in IHC?

I would like to stain for Epidermal Langerhans cell in rat skin. The problem is I am not sure what primary antibody would be the most appropriate for immunohistochemistry. I currently have CD103...

29 November 2017 4,291 3 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

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01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View