04 March 2021 3 7K Report

Hello,

If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange?

For example, if i flow through 500 uL of 1mg/mL sample, and collect the 700 uL of buffer elution, will the concentration of the elution always be less than 1 mg/mL? or will there be time when an antibody recovery after buffer exchange is 100% e.g. 1mg/mL elution

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