Which method better to extract the phytochemical from plant by different solvents starting from non polar solvent to high polar (Solid- Liquid Extraction) or do extraction first in 80 MeOH then do partition (Liquid - Liquid Extraction) for the crude extract in different solvents?
When I extract the phytochemicals, I usually use 100% Methanol 3 days for 3 times.
For medicinal plants, better to exctract using boiling water or ethanol/methanol-water mixture. Use this crude extract to further fractionation using solvents of different polarity for getting extracts enriched with the most bio-active compounds.
Alternately super critical carbondioxide extraction is best for extraction.
Use methanol first to obtain the crude extract, and partition it with Dichloromethane, ethylacetate and latter hexane.
Olajide Olutayo Dear sir thank you first for your answer "Use methanol first to obtain the crude extract, and partition it with Dichloromethane, ethyl acetate and latter hexane".
Sir How if i start partition with Hexane then Ethyl acetate, and late Dichloromethane because i see some papers following these order
Use methanol first to obtain the crude extract, and partition it with hexane, Dichloromethane, and ethylacetate
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