Hello can anyone tell me how to make complete medium with lhc-9 medium?? i want to start my work in beas 2b cell line..can anyone plz help me out/..how to make complete medium?? do we have to add fbs and antibiotic solution or not??
Use the basal media from stemcell technologies or the pneumaCult media; they work well. Or you can wait for the Lonza media, but that has a 5 month turn around.