How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What statistical test should I use if I want to compare values of temperature taken at aural, forehead and inner wrist ?

I basically want to see if there is a difference between these sites. Especially between the forehead and inner wrist as many patients are not very happy with temperature reading taken at the...

05 October 2020 3,151 12 View

What is the best way to determine if data collected by 3 instruments differs significantly?

Hello everyone, I am trying to statistically analyze whether data from 3 thermometers differs significantly. At the moment, because of COVID-19, several control points have come up at the...

04 June 2020 1,940 4 View

Is there a statistical Analysis for determining significant difference between data registered by thermometers?

Hello everybody, Is there, by any chance, a statistical method that might help me determine whether two thermometers' data average differ significantly from one another? Since our laboratory does...

17 May 2020 5,693 2 View

What is a good monitoring system for CO2 incubators?

I would like to monitor the environment within a CO2 incubator used for cell cultures. I am most interested in monitoring its temperature (37 C) and CO2 levels (5%). I would like to be able to get...

01 February 2020 8,899 2 View

Do we have a relationship between emissivity of material and temperature?

Not the radiative heat transfer (Q=sigma*A*e(Ta^4 - Tb^4)) equation. In other words, can we directly conclude, for a given emissivity this will be your temperature? Like the Infra Red...

05 December 2019 6,670 1 View

Is there any authomatic devise or method to measure a resistance from temperature dependence in semiconductors (VO2)?

For now, the method we use to measure resistance from temperature dependence in vanadium dioxide (VO2) is really slow: you need to look at oscilloscope and thermometer and write down the numbers...

28 June 2019 4,107 3 View

How much heat does an 75W aquarium heater immersed in oil transmit to a room of known dimensions?

I ran an experiment using model buildings approximately 0.068m3 in volume, with a 5l bottle of oil and a 75W aquarium immersion heater submerged in them. The temperature set point on the immersed...

31 December 2018 9,133 3 View

Band broadening in sequencing gels: How to avoid?

Hello all, I have been running sequencing gels for quite some time now and I find that the bands start broadening as the dye reaches the halfway mark (pic attached). I followed the publications...

10 November 2018 9,225 9 View

Band broadening in sequencing gels: How to avoid?

Hello all, I have been running sequencing gels for quite some time now and I find that the bands start broadening as the dye reaches the halfway mark (pic attached). I followed the publications...

03 November 2018 4,008 8 View

Are my adipose tissue samples still viable after being in a -80 that went down on us over the weekend?

I have some human adipose tissue samples. -80 C went down on us over the weekend. We are unsure of how long the freezer was actually down for. When we got it back on the outside thermometer on the...

07 August 2018 6,532 6 View